Kappa-casein gene polymorphism in Indian goats
نویسندگان
چکیده
The present work was carried out to analyse the κ-casein variants in five Indian goat breeds by SDS-PAGE, PCRRFLP and SSCP method. A total of 152 unrelated blood samples and 102 milk samples belonging to Barbari, Beetal, Marwari, Surti and Local MP (non-descript) goats were used for analysis. SDS-PAGE exhibited κ-casein allele A in all the breeds. Moreover PCR+RFLP analysis also confirmed the presence of AA genotype in all the breeds. PCR-SSCP analysis of κ-casein genetic variant showed the κ-casein A and B allele in the analysed samples. The κ-casein A allele was the dominant variant found in all analyzed breeds with frequencies ranging from 0.70 (Barbari) to 0.8 (Local MP). The variant B was most frequent in Barbari goats as compared to other breeds.
منابع مشابه
Genetic polymorphism and association of kappa-casein gene with milk production traits among Frieswal (HF × Sahiwal) cross breed of Indian origin
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